Martin Visser

3 Questions 4 Answers 0 Followers

Questions related from Martin Visser

How can I improve the attachment of my SF9 insect cells to tc flasks?

I have recently started working with SF9 cells and they have problems attaching to the TC flasks with less than 40% attached. I have also tried using poly-D-Lysine with little effect. In my...

06 May 2024 1,432 2 View

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

10 February 2021 3,911 6 View

Is there a method to remove the trypsin deactivating components of FBS in cell cultures?

I have transfected BSR5-T7 cell using TransIT LT1 from Mirus Bio, and 16hrs after transfection changed the Opti-Mem used in the transfection with full growth medium containing 5% FBS. Is there a...

02 February 2020 5,701 3 View