Martin Visser

2 Questions 3 Answers 0 Followers

Questions related from Martin Visser

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

10 February 2021 3,894 6 View

Is there a method to remove the trypsin deactivating components of FBS in cell cultures?

I have transfected BSR5-T7 cell using TransIT LT1 from Mirus Bio, and 16hrs after transfection changed the Opti-Mem used in the transfection with full growth medium containing 5% FBS. Is there a...

02 February 2020 5,685 3 View