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Questions related from Martin Sim
Hello fellow researchers! I think I have successfully generated a construct which should contain a piece of mRNA ligated to a ssDNA puromycin linker. I've done some gel mobility shift assays...
19 August 2019 2,094 0 View
Hi. Unfortunately our lab doesn't have a dark room, so when we are cutting bands from our Sybr-Safe or Midori Green stained gels, we often find it difficult to see the bands present which are...
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Hi. I'm looking in to doing somein vitro transcription from a linear dsDNA template, or a linearised plasmid. When my T7 RNA pol runs along generating the mRNA, once its hits the 3' end of the...
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