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Questions related from Martin Sim
I'm using the NAD/NADH Glo assay kit by Promega. I'm differentiating neuroblastoma cells into neurons using retinoic acid. I am able to get very long axons for axonal studies after a few...
31 May 2019 8,050 0 View
I'm using Rhod-2 AM to view calcium flux inside dying axons. During early hours of the treatment (4 and 6 hours), Rhod-2 AM stains the axons very brightly. But when I add Rhod-2 AM to dying...
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How vital is it to optimize the RNA input for reverse transcription for subsequent qPCR reactions? The qPCR reaction mix protocol calls for 25ng of template. I've been using 100ng total RNA for...
06 March 2018 1,675 4 View
I grow my neurons on glass surface. Their axons so delicate that I cannot remove the media entirely. When I refeed them, I remove half the media and replace it with fresh media containing double...
04 February 2018 1,967 3 View
It's my first experiment in flow cytometry. I'm attempting to exclude non-single cells. I'm wondering why it's not common practice to simply discriminate using pulse width (Time of Flight)? What...
29 November 2017 263 3 View
I grow my cells on both plastic Corning 96well culture plates for most experiments, but for immunoluorescence staining I'm growing them on glass or permanox chamber slides. One of the Chamber...
18 November 2017 8,255 3 View
The EF1 pCDH vectors by SystemBio use a composite promoter; hEF1a-HTLV http://discovery.ucl.ac.uk/20480/1/20480.pdf "hEF1a-HTLV promoter is a composite promoter comprising the human Elongation...
23 June 2017 5,297 1 View
