Martin Sim

8 Questions 2 Answers 0 Followers

Questions related from Martin Sim

Is it unheard of for the NAD/NADH ratio to completely flip?

I'm using the NAD/NADH Glo assay kit by Promega. I'm differentiating neuroblastoma cells into neurons using retinoic acid. I am able to get very long axons for axonal studies after a few...

31 May 2019 8,073 0 View

How does Rhod-2 AM aggregate in Mitochondria?

I'm using Rhod-2 AM to view calcium flux inside dying axons. During early hours of the treatment (4 and 6 hours), Rhod-2 AM stains the axons very brightly. But when I add Rhod-2 AM to dying...

21 May 2019 6,978 0 View

How vital is it to optimise total RNA Input for qPCR reactions?

How vital is it to optimize the RNA input for reverse transcription for subsequent qPCR reactions? The qPCR reaction mix protocol calls for 25ng of template. I've been using 100ng total RNA for...

06 March 2018 1,736 4 View

How would I optimise the concentration of Desipramine in Developing My Parkinson's Cell Model?

6-OHDA (Oxidopamine) and MPP+ are transported into the neurons via Dopamine transporters, as well as norepinephrine transporters (NET). At the moment, I'm focused mainly on 6-OHDA, in order to...

10 February 2018 1,016 2 View

How can you fix cells without removing media?

I grow my neurons on glass surface. Their axons so delicate that I cannot remove the media entirely. When I refeed them, I remove half the media and replace it with fresh media containing double...

04 February 2018 2,022 3 View

Why can't we discriminate singlets using Width (Time of Flight) only?

It's my first experiment in flow cytometry. I'm attempting to exclude non-single cells. I'm wondering why it's not common practice to simply discriminate using pulse width (Time of Flight)? What...

29 November 2017 307 3 View

Can you perform fixation and immuno fluorescent staining in plastic 96 well plate tissue culture plates?

I grow my cells on both plastic Corning 96well culture plates for most experiments, but for immunoluorescence staining I'm growing them on glass or permanox chamber slides. One of the Chamber...

18 November 2017 8,290 3 View

Has anyone had problems with promoter methylation using pCDH vectors?

The EF1 pCDH vectors by SystemBio use a composite promoter; hEF1a-HTLV http://discovery.ucl.ac.uk/20480/1/20480.pdf "hEF1a-HTLV promoter is a composite promoter comprising the human Elongation...

23 June 2017 5,340 1 View