Martin Oheim

3 Questions 8 Answers 0 Followers

Questions related from Martin Oheim

Is anyone aware of a measurement of the fluorescence quantum yield (Ca-free/Ca-bound) of X-rhod1?

In order to calculate the molecular brightness (product of molar extinction coefficient and fluorescence quantum yield) I would like to know that number. Thanks a lot in advance.

16 January 2014 2,086 1 View

Has anyone tried fast photomultiplier tube (PMT) blanking to protect photomultipliers from intense light in experiments combining optogenetics and 2PEF microscopy?

We tried to cut the HV across the dynodes with a fast switching relay (which works), but we get a strange square after-pulse (which is much longer than the electron transit time across the PMT),...

20 February 2013 2,912 2 View

Does anyone have experience in designing a fast (couple of ms) blanking circuit for protecting photomultiplier tubes from overload?

We tried cutting the HV (and electron amplification) with a fast HV relay, but have a strange and, as of yet, unexplained square after pulse with stereotyped length and an amplitude depending on...

19 February 2013 1,533 1 View