Markus Schäfer

2 Questions 4 Answers 0 Followers

Questions related from Markus Schäfer

Does anyone know if the insertion site of the mouse OT1 transgene has ever been mapped?

Hi,does anyone know if the insertion site of the mouse OT1 transgene (C57BL/6-Tg(TcraTcrb)1100Mjb/J or https://www.jax.org/strain/003831) has ever been mapped?Would be nice to have a het vs hom...

25 July 2016 6,689 0 View

Does anyone have experience with (Amaxa Nucleofector - based) transfection of murine hematopoietic progenitor cells?

Hi! I want to transfect murine hematopoietic progenitors with a Cas9-encoding plasmid (so the thing is quite large). I want to use Pax5- or Ebf1-deficient cells, either freshly isolated and in...

04 August 2014 7,840 7 View