Mark Slayton

2 Questions 8 Answers 0 Followers

Questions related from Mark Slayton

How do I prevent over-salting of the genomic DNA pellet during post-extraction purification/precipitation?

I have extracted total DNA from human diploid fibroblasts grown on the surface of 60mm dishes. Everything looks good, but when I completed successive phenol:chloroform extractions and then added...

06 June 2014 5,790 15 View

Difficulties with PCR on a region of the HCMV ori which contains many mononucleotide repeats - can anyone help?

I'm working with a plasmid that contains a 5.1kb insert of HCMV DNA (total size is ~8kb). Initially, I attempted site-directed mutagenesis on the entire plasmid but I could not obtain a product....

10 October 2013 9,785 1 View