Mariya Y. Radeva

5 Questions 8 Answers 0 Followers

Questions related from Mariya Y. Radeva

Direct transfer of insert from one pDEST into another pDEST vector-is it possible?

Hi guys, I guess it is not that clever idea to try already cloned sequence into pDEST-EGFP-N1 vector to be “extracted” from the vector by restriction and inserted into pDEST-EGFP-C1 vector? Or I...

10 October 2019 6,660 1 View

Make statistic with less than 3 patients per group?

Hi there, I probably know the correct answer, however, I would appreciate if you guys share with me your opinion whether is accurate to do any statistic when less than 3 donors are available per...

02 February 2018 8,497 3 View

Plasmid isolation of certain insert failed-why?

Dear colleagues, I try to insert 2 different PCR products (relatively the same size, apprx 3000 bp) to pCR 2.1.TOPO vector. For this purpose I add poly A to each PCR product. Consequently the...

07 July 2017 7,819 3 View

Which is the way to clone big inserts (9000 bp)?

Hi there, I will appreciate if someone gives me an advice how to clone big inserts in range of 9000 bp...I tried with Gateway system- does not work! best wishes Mariya

10 October 2015 1,737 6 View

How to perform PKA activity measurements?

Can anyone recommend a good kit for PKA activity measurements (based on his/her own experience.) If possible, non-radioactive. Thanks in advance!

02 February 2014 2,849 3 View