Lipofectamine 2000:DNA complexes are stable for up to 6hrs. Has anyone tried incubations of > 15 min on lipofectamine3000:DNA complexes?

Transfecting HEK 293A cells. I've done lipofectamine 2000 transfections with incubation times between >20 min, < 4 hrs and they worked well. According to manufacturer they should be stable...

06 April 2016 4,303 1 View