8 Questions 10 Answers 0 Followers
Questions related from Marilia Cavalcanti
Someone would have some reference of Clostridium spp primer for qPCR to indicate? I'm looking for one that has less than 500 bp. I already used the sequence: F 5′-AAAGGAAGATTAATACCGCATAA-3 ′ and R...
01 January 2020 6,780 3 View
I am analyzing an intestinal microbiota of rats by qpcr. I had already done the efficiency of my primers and analyzed samples of DNA extracted from feces and everything went well. Good melt curve...
01 January 2020 673 3 View
Usually the melting cycle of 95 ° C for 15s, 60 ° C for 60s and 95 ° C for 15s is used. In my laboratory, they normally used an annealing temperature of 60ºC, I intend to change it to 61.5ºC and...
01 January 2020 8,278 3 View
I am working with DNA extracted from feces. I made a qPCR using concentrations of 100, 75, 50 and 20ng final. The CT value was very similar between dilutions between 29 and 30. My positive control...
11 November 2019 7,919 8 View
The picture of gel is there
05 May 2019 238 4 View
I already used a lysis buffer, but it did not help.
04 April 2019 5,619 3 View
I will perform dna extraction from feces and kits are very expensive. I saw that extracting DNA from feces and reading PCR is a bit problematic. Has anyone used any adapted protocol? With lizosima...
02 February 2019 7,598 1 View
I am trying to extract bacterial DNA from feces and cecum from mice, to then do PCR. I initially tried with phenol-chloroform and did not succeed. I had better results with CTAB, but the DNA band...
01 January 1970 6,658 3 View
