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Questions related from Marilia Cavalcanti
I am evaluating histological slides (H&E) of brown adipose tissue from rats. And counting the number of unilocular adipocytes to evaluate the whitening process. How to identify? Would it...
14 May 2024 7,841 0 View
How to count multilocular adipocytes on a slide with a white adipose tissue sample stained with H&E? I had done the count on the entire slide, but the articles put it in % of cells or...
11 April 2024 3,967 2 View
Have you ever used a syringe/needle method for RNA extraction with Trizol? It would be in the homogenization stage. I read in an article that it would be better than homogenizing with beads....
14 February 2022 9,923 3 View
The website "themicrobiome.com" had the image of the 16S gene and in each region showed sequences of primers. This site has been offline for a few years. I wanted to consult this bank of 16S...
13 January 2022 5,452 1 View
What would be the acceptable CV% and SD for copy number results between stool samples in qPCR (same group)? I am evaluating different bacteria (E.coli, Lactobacillus, etc.) using the absolute...
07 August 2020 8,256 4 View
I already tested three different sequences and had problems with dimers and melt curve (low specificity). Any reliable primers? I'm studying rat microbiota with qPCR.
13 March 2020 7,775 2 View
Someone would have some reference of Clostridium spp primer for qPCR to indicate? I'm looking for one that has less than 500 bp. I already used the sequence: F 5′-AAAGGAAGATTAATACCGCATAA-3 ′ and R...
20 January 2020 7,288 3 View
I will perform dna extraction from feces and kits are very expensive. I saw that extracting DNA from feces and reading PCR is a bit problematic. Has anyone used any adapted protocol? With lizosima...
08 February 2019 2,297 1 View
