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Questions related from Mariano De La Piedad Marín-Blazquez Hervás
Hello, We are designing a protocol to determine the effect of certain compounds in the health of rats, but our problems have certain instability issues when subjected to certain pHs. We were...
20 June 2024 5,818 1 View
I am trying to establish a model of hypoxia-reoxygenation in HK-2 cells in order to determine their expression of kidney injury biomarkers (e.g. KIM-1, NGAL, etc.) via qPCR. However, I am finding...
10 May 2024 9,198 0 View
I am currently trying to develop an ATP concentration measurement protocol for my human cultured cells. However, I have found little to no detailed protocol for ATP extraction using TCA...
28 November 2023 7,953 1 View
I am currently working with HK-2 renal cells from the ATCC. To this day, cells have been growing regularly and rapidly, but since I started using pen/strep for my cultures, cells have been stuck...
27 October 2023 5,377 0 View
I am aware that immunohistochemistry is a qualitative or semi-quantitative technique, but I performed several anti-CD163 immunohistochemistries in order to study the presence/absence of M2...
05 April 2023 3,082 1 View
