Marianne Ariganello

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Questions related from Marianne Ariganello

What is the minimum fractionation method that is needed for whole mammalian cell lysate prior to Orbitrap-MS?

Hello, I am new to proteomics and we are designing some experiments to look at proteomic profiles from cell lysates. Our previous MS method needed a more simplifed mixture of proteins, so we had...

02 February 2015 10,041 5 View

How do you dry your samples for Proteomics?

We are using MWCO to fractionate our cell samples, then we evaporate our samples (to concentrate them) prior to digesting with trypsin. We have typically used N2 to dry the samples (inert...

14 August 2014 2,079 14 View