Marianne Ariganello

4 Questions 7 Answers 0 Followers

Questions related from Marianne Ariganello

What is the minimum fractionation method that is needed for whole mammalian cell lysate prior to Orbitrap-MS?

Hello, I am new to proteomics and we are designing some experiments to look at proteomic profiles from cell lysates. Our previous MS method needed a more simplifed mixture of proteins, so we had...

02 February 2015 10,119 5 View

How do you dry your samples for Proteomics?

We are using MWCO to fractionate our cell samples, then we evaporate our samples (to concentrate them) prior to digesting with trypsin. We have typically used N2 to dry the samples (inert...

14 August 2014 2,129 14 View

Can someone tell me if my understanding of enzyme inhibition assays is correct?

Here is my thought process: To quantify the activity of an enzyme (ENZ) I look at how it cleaves the classical substrate A-B, and measure the cleavage product B. Now I also have a competitive...

31 May 2014 1,928 5 View

What is known about SPARC (osteonectin) expression in macrophages (specifically U937)?

I'm performing some immunofluorescence for SPARC (osteonectin) in PMA differentiated U937 cells. I am wondering if anyone has looked at SPARC expression in macrophages and U937s in particular,...

04 May 2014 7,646 2 View