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Questions related from Marianne Ariganello
Hello, I am new to proteomics and we are designing some experiments to look at proteomic profiles from cell lysates. Our previous MS method needed a more simplifed mixture of proteins, so we had...
02 February 2015 10,117 5 View
We are using MWCO to fractionate our cell samples, then we evaporate our samples (to concentrate them) prior to digesting with trypsin. We have typically used N2 to dry the samples (inert...
14 August 2014 2,125 14 View
Here is my thought process: To quantify the activity of an enzyme (ENZ) I look at how it cleaves the classical substrate A-B, and measure the cleavage product B. Now I also have a competitive...
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I'm performing some immunofluorescence for SPARC (osteonectin) in PMA differentiated U937 cells. I am wondering if anyone has looked at SPARC expression in macrophages and U937s in particular,...
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