Mariana Franco Fragoso

3 Questions 6 Answers 0 Followers

Questions related from Mariana Franco Fragoso

Do any of you have experience with scratch assay in Caco-2 cells?

I'm having a lot of trouble trying to do the scratch, because patches of cells come out and then I don't have a perfect line for the assay. I used a p200 tip and washed with PBS after the scratch...

10 October 2015 9,183 5 View

Is there a way to look for reference genes in my NanoString miRNA data?

I've been reading about NormFinder and BestKeeper, but I'm not sure they can be used for miRNA. If so,how can I do that? Do you have any other suggestion about this?

05 May 2015 2,623 1 View

What are the best controls for miRNA normalization in human serum samples?

I just started working on miRNA and I still have a lot of doubts about them. I'm going to validate some miRNA expression using TaqMan miRNA Assay (Life) in serum from mice and human. My question...

05 May 2015 2,329 3 View