Mariana Aguilar

4 Questions 3 Answers 0 Followers

Questions related from Mariana Aguilar

Does anyone have a protocol for alkylation of samples using acrylamide prior to SDS PAGE analysis?

Hi! I'd like to try the protocol described by Mineki et al., but I'm confused about the ratio of acrylamide : protein concentration in order to achieve proper alkylation of my free thiols and...

20 September 2024 9,758 0 View

Help with SDS NR vs Native Page: how is it possible to get greater bands of the same sample in native page?

I'm working with both Native PAGE gels and SDS under non reducing (SDS-NR) conditions gels. My understanding is that Native PAGE would resolve "native-like" proteins, and the SDS-NR bands would...

19 September 2024 3,680 2 View

How to obtain the standard deviation of multiple sample scans in FTIR ATR Cary 630?

Hello! Does anyone know where can I find the standard deviation out of the multiple scans done in FTIR ATR using Cary 630? For my method, I've selected 64 sample scans, so as to improve the signal...

03 April 2024 9,739 2 View

Any thoughts on why I'm getting the same DTA+ TGA thermograms out of two different standards?

I'm working with TGA (Thermogravimetric analysis) + DTA (Differential thermal analysis) of milk proteins on a STA2500 Regulus equipment by Netzch. I have two standards: one of B-Lactoglobulin and...

19 January 2024 2,447 1 View