Maria Simanovich

7 Questions 16 Answers 0 Followers

Questions related from Maria Simanovich

Al-trans retinoic acid handling. How to handle?

Hi everyone. I'm going to work with ATRA. My question is: which precausion do you use when working(i see it is quite dangerous especially for women). And how do you dispose it and everything it...

18 March 2024 5,828 0 View

High Capacity cDNA Reverse Transcription Kit why the extension time so long?

Hi everyone. For my project I have to reduce the time for reverse transcription of mRNA. We are using High Capacity cDNA Reverse Transcription Kit from Applied Biosystems. It's recommended there...

16 June 2021 8,251 3 View

Ni-NTA based protein purification RAD51. I don't see any peaks, why?

I'm working wiht RAD51-6HIS using Ni-NTA column i'v got the following chromatogram (attachement). There are almost no peaks. I haven't done SDS page yet but, I want to do Ni-NTA one more time...

01 January 1970 4,079 14 View

U2OS clones after transfection with lipofectamine 3000

Hi everyone, I'm currenctly working on the project CRISPR CAS9 transfection of U2OS cells with lipofectamine 3000. The question I have: why after transfection different cel lines produce differen...

01 January 1970 658 3 View

Making empty vector from pGBW-m4046887

Hi, i'm trying to cut the SARS-COV2 S protein from the plasmid pGBW-m4046887 to get een empty vector with small 6-His tag protein which I want to use for negative selection for SELEX. I did PCR...

01 January 1970 4,793 4 View

Protein knockout confirmation: good on WB&qPCR, no changes in the sequence.

Hi everyone! I've used clustered regularly interspaced short palindromic repeat (CRISPR/Cas9) gene editing approach to knock out one gene. The knock down was performed via lentiviral transduction...

01 January 1970 4,133 5 View

Sh-RNA seems to be working on WB, but not really on qPCR. Why?

Hi everyone. I'm trying to knock-down COUP-TFII in K562 cells using short-hairpins. I'm using viral transduction. I have 5 construct and on WB (day 4 after transdcution) it looks goed. Also the...

01 January 1970 156 3 View