Maria Russo

2 Questions 10 Answers 0 Followers

Questions related from Maria Russo

Does anyone have an idea how to remove the background signal in Western Blot due to the IgG immunoprecipitates when MW of my protein is 50-60 kD?

Technical problems with protein immunoprecipitation.

10 October 2013 4,335 1 View

Has anyone had problems in interpreting the results of a WB on U2Os using LC3B antibody?

Let me know if any of you have ever used an antibody LC3 (code 2775 Cell Signaling) in U2OS cells to monitor autophagy. The two bands that can be seen around 15 kD are LC3 II?

01 January 2013 7,377 4 View