Maria Lorena Oróstica

3 Questions 6 Answers 0 Followers

Questions related from Maria Lorena Oróstica

Why the medium for transfection with lentivirus in cancer cells do´t use antibiotics?

In some protocols for some cell types I have seen that don´t use antibiotics for this technique. But I don´t know why. Thanks in advance

04 April 2017 7,653 0 View

MiR controls in qRT-PCR?

I was wondering which are the SYBR green miR controls that are usually used? I'm using RNU6-B but I don't get the same Ct values on my samples, even if they are cultured samples. I'm using...

05 May 2016 5,091 2 View

Does someone work with endometrial cell lines, e.g. HESC or St-1?

I work with both lines, but it is very difficult to expand them under standard conditions.

03 March 2015 679 4 View