Maria Margarida Mesquita

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Questions related from Maria Margarida Mesquita

Can anyone suggest a density gradient centrifugation media to purify Bacillus subtilis spores?

We need a density range of 1.2-1.4 g/mL

04 April 2016 9,929 3 View

Does anyone have experience using the a Anatoxin-a RBA ELISA kit?

We need to quantify anatoxin-a in drinking water from a reservoir. Has anyone tried the Abraxis anatoxin-a RBA ELISA kit for quantification of anatoxin in water samples? If so, what has your...

09 September 2014 3,132 0 View

Does anyone have a good method for bacterial cells staining with acridine orange for epifluorescent microscopy counting?

We are having trouble enumerating our Shewanella cells by epifluorescence microscopy after staining with acridine orange, mainly because the cells tend to clump together and are so small we can...

03 March 2014 9,866 0 View

Can anyone suggest a reliable method for DNA extraction from sand?

With the Powersoil extraction kit we get high DNA concentration readings and high contaminant ratios by UV-Vis spectrophotometry. However we can not visualize the DNA in agarose gels (1% or 0.7%).

09 September 2013 9,644 10 View