Maria Ciria

6 Questions 25 Answers 0 Followers

Questions related from Maria Ciria

Hi! Someone can help me with SUMO1?

Hi!! Someone know best method to analyze SUMO1 by western blot? I tried different buffers to extract proteins, different loading buffers, different antibodies and doesnt work :S:S I'm getting mad...

02 February 2016 3,614 6 View

Can someone help me with absolute quantification of miRNAs (TaqMan PCR)?

Hi!! Someone can help me? I'm looking for an analysis to quantify absolute miRNAs in a PCR using TaqMan. Not a relative quantification vs C.elegans mir39 or vs an endogenous miRNA. Thanks so...

09 September 2015 541 10 View

Does anyone know a cheaper way to do notch activation?

Hi! I'm using Jagged1 ab to stimulate Notch pathway in a cell line...but it is so expensive at the end...so, someone know how I can do it cheaper? I mean, other product? other conditions? Jag...

09 September 2014 4,146 2 View

What is the best pH buffer solution?

Does anyone know what is the best buffer to keep on the pH on a medium (complete and KH) with Nigericin and Monensin to permeabilize our fibroblasts and detect through FITC-dextran their...

10 October 2013 5,373 4 View

What is the best method to determine the redox state of a protein? Could it be done using SDS-PAGE and adding, for example, DTT?

02 February 2013 1,417 6 View

Does anyone know a method or protocol to isolate lysosomes, possibly by density gradient or something like that?

Isolate lysosomes.

02 February 2013 7,167 3 View