Maria Carolina Bittencourt Gonçalves

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Questions related from Maria Carolina Bittencourt Gonçalves

What is the best/easiest way to measure lithium levels in blood samples from an animal model for bipolar disorder?

I'm working with animal models for bipolar disorder to investigate lithium mechanisms of action. However i've been using lithium dosage based only in the suggestions from the literature. Indeed, I...

04 April 2016 8,526 4 View

Is it essential to bubble aCSF with CO2 5%/O2 95%? How to do it in a easy/cheap way?

I'm planning to prepare aCSF for the first time but I dont know exactly how to bubble the CO2. As I saw here in Q&A a lot of people was having problems with pH, what was suggested to be...

05 May 2015 9,229 0 View

Is it okay to homogenize brain tissue with ultrasonic waves before following TRIzol's protocols for RNA, DNA and protein extraction?

I need to isolate RNA, DNA and protein from lots of samples of hippocampus and pre-frontal cortex obtained from adult rats. Would there be a problem if I were to use ultrasonic waves to homogenize...

10 October 2014 4,995 7 View