How to supplement M9 media for E.coli growth?

I am growing E.coli in M9 supplemented media (M9 salts, 1mM thiamine hydrochloride, 0.4% glycerol, 0.2% casamino acids, 2mM MgSO4 and 0.1mM CaCl2). However, the growth is not as good as in LB...

29 November 2019 3,211 6 View