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I am imaging cells which have endogenous GFP signal and I stained them with DAPI in mounting medium. I use confocal ZEISS LSM800. Some of the cell soma (looks like cytoplasm and processes) seems...
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Does anyone know how should I perform experiments in ScaleS protocol for tissue clearance? I have fully dissected brain, and what next? Should I first clear it out with ScaleS protocol and then...
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Hello! I am looking for the working protocol, which I could use for clearing the brain section, 105 µm thick (hemisphere). Without this I am not able to reach deeper into the tissue than 20...
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Dear All, Could anyone help me with 3D analysis in software ICY? I cannot render all cells which are in my z stack in 2 channels, it shows only part of blue channel, but green one, in which cells...
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