Maria Ciria

5 Questions 17 Answers 0 Followers

Questions related from Maria Ciria

Hi! Someone can help me with SUMO1?

Hi!! Someone know best method to analyze SUMO1 by western blot? I tried different buffers to extract proteins, different loading buffers, different antibodies and doesnt work :S:S I'm getting mad...

17 February 2016 7,772 5 View

Hi!!! Anyone have a protocol to extract proteins to analyze SUMOylation by WB??

Hello! I'm looking for a protocol to extract proteins just to analyze SUMOylation by Western Blott. I don't want to analyze the SUMOylation of a target protein, just degree of sumoylation in...

08 September 2015 6,024 4 View

How can I measure the activity of gamma secretase?

I'm looking for some accurate assay to measure the activity of gamma secretase by colorimetric approaches and really, I can't find any kit. Does anyone know how I do it? Or where can I find...

11 March 2015 5,874 7 View

How do I analyze a time lapse assay?

Hi! I'm doing a time lapse assay to see invasiveness of different kind of cells. Anyone know how to measure it with ImageJ? Thanks in advance!!

15 December 2014 1,226 3 View

Does anyone know a cheaper way to do notch activation?

Hi! I'm using Jagged1 ab to stimulate Notch pathway in a cell line...but it is so expensive at the end...so, someone know how I can do it cheaper? I mean, other product? other conditions? Jag...

18 September 2014 430 2 View