Maria Arshad

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Questions related from Maria Arshad

Why I am keep observing reduced growth of MDA-MB-231 cells in phenol red free RPMI media as compared to regular RPMI media?

I am using gibco RPMI Medium 1640 (1X), [+] L-glutamine, [-] Phenol Red with 10% FBS to grow the MDA-MB-231 (triple-negative) cancer cells in 5% CO2 and 37c temperature. I always seed 20%,...

25 September 2021 3,664 3 View

What assay measures the size of the transcription bubble?

You are studying the function of phosphorylation of TFIIH with extracts from yeast. You can add these extracts to dsDNA and have transcription occur in vitro (if you also add rNTPs). Your research...

04 March 2019 965 1 View

Cell cycle arrest in MDA-MB-231 and MCF-7 under same FEC treatment and conditions.

MDA-MB-231 which is a triple negative BC cell line showed significant cell cycle arrest at the S phase and also at the G2/M phase. On the other hand, MCF-7 which is an ER-positive BC cell line...

01 January 1970 5,625 0 View