Margarette Clevenger

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Questions related from Margarette Clevenger

Help with FACS voltage setting for FSC v SSC?

I am new to flow cytometry and have not had formal training on setting appropriate voltage / (gain?) values. I ran PBMCs isolated from whole blood, and tried to adjust FSC and SSC values so all my...

02 August 2017 1,445 4 View

I am trying to biotinylate cells, and then treat them with Streptavidin conjugated to Alexa488 in order to visualize under IF. Not working, thoughts?

I am biotinylating with Sulfo-NHS-LC-Biotin, dissolved in PBS (1 mg / mL) changed to a pH of 8. I added biotin for 30 minutes on ice to a layer of endothelial cells plated on a collagen matrix. I...

30 May 2017 8,065 10 View