Marcus Bezerra

3 Questions 3 Answers 0 Followers

Questions related from Marcus Bezerra

What is the percentage of positive cells from a stable CHO pool after months of selection?

I have been generating stable cell clones (antibody production) using the ExpiCHO system + antibiotics selection. Although I have been able to select a few producing clones after months of...

11 August 2024 6,159 1 View

Why is my SUMO-tagged protein being degraded after purification?

I express antibody fragments using SUMO tag, in E. coli. I have been able to get purified proteins after couple steps of chromatography, but there is a very strong band of lower size after IMAC....

14 February 2023 7,729 4 View

Does imidazole influence in flow cytometry for antibody fragments?

I am screening several scFv sequences by flow cytometry. I got good expression/purification yields and next step is dialysis to remove imidazole and then flow cytometry to see if they bind to...

15 September 2022 5,305 0 View