12 Questions 33 Answers 0 Followers
Questions related from Marco Paoli
Has anyone ever used viruses to express fluorescent proteins in living insects?
02 April 2013 2,940 1 View
I am trying to perform immunohistochemistry on the whole brain of bees using antibodies anti-Repo and anti-Elav (from DSHB, Iowa) but with very little result. Has anyone experience with one of...
07 March 2013 7,686 0 View
What would you advise for in-vivo two photon calcium imaging between Fura-2 and Oregon Green 488-BAPTA? I cannot do ratiometric analysis because the set-up does not allow it. Any suggestions?
29 January 2013 2,764 7 View
Is it possible to selectively "burn" some regions with a laser pulse from a two-photon microscopy? I would like to selectively damage some brain regions in insects in order to study neuronal...
23 January 2013 9,424 4 View
Is there anyone who has tried to label cells with Hoechst or similar in living invertebrates? I am thinking about labeling the nuclei in the brain of a living insect. Has anyone ever tried it?
22 January 2013 5,791 17 View
I am working on a non-model animal and I am searching for in vivo labelling tools. In particular I would like to label cell nuclei (mainly astrocytes and neurons). Is there any specific or cell...
16 January 2013 2,517 21 View
Invertebrates have also glial cells but they are a bit "different" and less specialized than the one we can find in mammals. Does anyone know if markers such as GFAP, EAAT-1, EAAT-2, GLUTAMINE...
31 October 2012 2,764 5 View
Does electroporation efficacy change when electroporating axons rather than soma? Is it possible to target a pupulation of neurons by electroporating their axons (which are all bundled together)?...
29 October 2012 3,038 7 View
I would like to measure a possible change in synaptic coverage by astrocytes upon memory formation. Do you think there is some way to measure it by IHC and optic microscopy (mainly 2-photon...
15 October 2012 2,482 5 View
Is it possible to highlight insect neurons with flurescent protein-expressing virus? Has it ever been done?
05 October 2012 130 6 View
is it doable to intra-venous inject cell imaging dyes such as sr101 or RH795?
04 October 2012 4,192 4 View
How would you stain glial cells in bees for in vivo imaging? Would SR101 work?
04 October 2012 4,310 3 View
