Marco Bravo

4 Questions 3 Answers 0 Followers

Questions related from Marco Bravo

EMSA free DNA and protein titration makes little sense, what could be going on?

Hello, has anyone had issues with EMSA data making little sense and rando behavior, I have recently been trying to run EMSAS of a 62Kda protein, with a High PI of 9.09 and I am experiencing very...

last month 6,135 1 View

Thin DNA gel loading sample tips?

I have a 0.25mM thick gel and I am having trouble cleaning out the wells to ensure constant sample application. I usually use a thin syringe to get into the wells and rinse them out with running...

02 July 2024 2,309 2 View

Mini-prepped Plasmid Uncut DNA runs way higher than cut DNA ?

Hello all, I have a mini-prepped DNA that I am trying to digest with EagI. After doing the digestion it looks like the reaction with the restriction enzyme maybe linearizing my plasmid as it...

09 May 2024 1,202 3 View

Does anyone know what could be going on with my cloning methods?

I am currently trying to generate expression constructs of truncated proteins for x-ray crystallography. It is my first time doing cloning. I have had success so far in PCR amplifying my inserts...

20 April 2024 6,125 5 View