Marcin Chrusciel

4 Questions 6 Answers 0 Followers

Questions related from Marcin Chrusciel

No amplification curve but suitable melting curve after qPCR. What could be the reason?

Analyzing qPCR (Sybr-green) results from one of my last experiments I found that for few samples there was no amplification curve, however specific melt peaks and sharp bands on the gel were...

07 July 2014 9,125 1 View

Could anyone recommend (have good experience & performed there) a good center for microarray analysis?

We want to do microarray with a reasonable/moderate price (either as collaboration+payments) or as service. Hybridization of 32 or 36 mouse samples by microarray analysis (without bioinformatics...

10 October 2013 1,028 5 View

How long can powdered bovine insulin be stored at -20C?

I found unopened bottle with powdered insulin (100 mg) bought in 2008 and stored until now at -20C. Is it still biologically active or should I buy fresh. I would like to use it to supplement the...

05 May 2013 5,604 7 View

What is the best method to remove dead cells from hybridioma cell suspension?

Usually 3 methods are recommended 1. Ficoll-hypaque 2. Wash 3x times with medium. 3. Percoll density gradients. however, I have no idea which protocol will be the best for sensitive hybridioma...

03 March 2013 1,263 9 View