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Questions related from Marcia Chavez
I have these squiggles that are appearing in my GAD67 IF stain and I'm not sure why. I've used this secondary antibody (Cy5 goat anti-mouse) with another primary antibody and never had this...
27 June 2022 2,038 9 View
Can the rolling ball radius tool be used alone to reduce background in fluorescent images? Why or why not? If not, what are some additional methods to further remove background after rolling ball...
24 February 2022 3,831 3 View
I'm looking for alternatives to Minocycline that would be relatively safe to administer during pregnancy in a rodent model. So far, I've only found one paper that gives this drug prenatally and no...
05 November 2021 435 1 View
Aside from embedding brains in an OCT block, are there other ways to section multiple brains at once?
19 June 2021 3,993 4 View
I recently tried an ERa immunofluorescent stain using strep488 as the secondary. There are clearly ERa positive cells, but I'm not sure what these "dots"/noise are in the background of my GFP channel.
22 March 2021 7,039 10 View
I've been troubleshooting my IF ERα protocol and I'm curious to see what reagents other people use in this stain. Thanks.
01 March 2021 4,525 1 View
I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...
08 February 2021 7,639 6 View
I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...
11 November 2020 3,725 7 View
