Marcel Zimmermann

4 Questions 4 Answers 0 Followers

Questions related from Marcel Zimmermann

Troubleshooting Ultraview Confocal microscope: what could be possible reasons for strange ripples or Moiré-like patterns in the DIC (brightfield)?

We observe strange wave-like patterns in our DIC (brightfield) image of our Perkin-Elmer ultraview confocal microscope. These ripples/waves do not show up in the confocal laser images, but only...

07 September 2021 1,900 2 View

How can you do statistics with normalized data?

Just briefly: I have WB quantifications from 4 different blots (4 biological repilcates) and I normalized my signal intensity values to the untreated ctrl sample. Therefore, for all 4 replicates...

15 July 2019 3,466 3 View

Internal normalization in Western Blot quantification and statistics. How to?

This might seem like a rather simple problem, but I am actually unable to find a helpful solution that specifically refers to this problem. I did several Western Blots of biological replicates...

11 July 2019 6,689 5 View

Method for fast Western Blot Transfer?

Hello, we are currently using semi-dry WB transfer systems from Bio-Rad (SD Transfer Cell) with a maximum Voltage of 25 V. Additionally, we limit the current to 60 mA per 6x8 cm gel (roughly 1.2...

07 March 2019 3,695 9 View