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Questions related from Marc Paul O'Sullivan
Can anyone recommend a good statistical test or model for examining differences in M-values or beta-values across multiple time points for a single patient?
29 August 2019 5,761 0 View
We know that extracellular vesicles and protein bound RNA is stable in the circulation. Can anyone recommend why cell-free DNA doesn't degrade in the circulation?
29 March 2019 5,238 3 View
Can you perform a log transformation, i.e. Log2 on your 2^-DDCT data to reduce the skew, thereby performing parametric tests as these may be more robust and reliable than non-parametric tests.
07 March 2019 7,360 3 View
Certain protocols call for serum to be spun twice, sometimes the second time is at a higher rate. Can anyone give me a technical explanation for: 1) Why a second time? 2) Why at a higher rate? I...
14 February 2019 5,795 4 View
To my knowledge the main way to extract white blood cells from a blood sample is spinning down an EDTA tube and carefully taking the plasma layer off, and trying to remove the buffy coat, which...
14 August 2018 3,608 2 View
With some kits, you make up 20ul of cDNA, and you can store this cDNA at -20oC. Then in the PCR step, you are asked to dilute 5ul of this cDNA by 80X and are recommended not to store it at this...
14 March 2017 2,472 3 View
I've been working with the SN48 as my housekeeper for my RT-qPCRs, this seems like quite a stable housekeeper as it remains around 22-24 CT's consistently throughout the runs across different...
13 February 2017 9,769 3 View
Samples are extracted from tempus tubes.
05 May 2016 5,472 2 View
