Which is the ideal B27 concentration for culturing primary cortical neurons: 2% b27 (50x) or 2% b27 (1X)?

The culture medium that i use is: -Nuerobasal -B27 2% -Glutamax o.5 mM -Pen/strep 2% However i dont know the conectration of the 2% of b27, should it be 50X (as it comes from the bottle) or 1X

01 September 2017 2,079 4 View