Manoj Thakur

3 Questions 12 Answers 0 Followers

Questions related from Manoj Thakur

How to avoid precipitation of a protein for ITC experiments?

Hello all, I am working with a protein X for which we are running an ITC (Isothermal Titration Calorimetry) experiment with a ligand ATP. When we have performed the initial experimental...

14 October 2021 6,383 3 View

Does anyone know how to prepare stock solution of bathophenanthroline for experiments?

I tried with DMSO(100%) and 50% ethanol but bathophenanthroline didnot appeared soluble. Does any one have any idea how to make 100 mM solution of Bathophenanthroline?

15 January 2018 8,538 2 View

Decrease in fluorescence anisotropy in the presence of protein, so how to explain it?

Dear all, Am studying protein-DNA interactions, in which ssDNA is labelled at 3'end with 6FAM(fluorophore). After incubating 0.5nM of this labelled DNA with increasing concentration of my protein...

06 September 2016 854 9 View