Manish Gore

3 Questions 7 Answers 0 Followers

Questions related from Manish Gore

How to resolve issue background fluorescence during Immunofluorescence (IF) studies of Hacat cells and Choroid Retinal Endothelial cells (RF/6A)?

Hello, I am studying cytokeratin 14 (K14) expression in Hacat human keratinocyte cell line and also von Willebrand factor (vWF) expression in choroid retinal endothelial cells (RF/6A). RF/6A cell...

10 October 2019 1,655 4 View

How to maintain the level of Matrigel loaded cells in 3D culture using Microfluidic Chip?

I am using Matrigel for 3D organotypic co-culture of mammalian cells on microfluidic chip. I am using thick gel protocol of Matrigel for the same in order to entrap the cells. Initially I had...

01 January 1970 6,109 0 View

How to perform H and E staining of cryosectioned spheroids?

We are developing monoculture spheroid model. Spheroids are developed in Ultra low attachment round bottom well plate and transferred to OCT cryomold and cryosectioned. Cryosections are of 5...

01 January 1970 3,713 6 View