Manish Gore

5 Questions 4 Answers 0 Followers

Questions related from Manish Gore

Has anyone conducted tube formation assay for RF/6A cells?

We have tried conducting tube formation assay for RF/6A (Choroid Retinal Endothelial Cells obtained from Rhesus species). Passage number is 46. We have used MEM (ATCC recommended) medium...

27 May 2019 2,187 0 View

Is there any working light sheet fluorescence microscopy facility in India?

I wish to do light sheet microscopical imaging of 3D Spheroids. I am looking for such facility in India. The system at NCBS isn't functional right now. Please let me know if anyone is aware about...

08 April 2019 769 0 View

Why do I get clumps while culturing primary human dermal fibroblasts in 3D in matrigel?

Hello everyone. I am trying to achieve 3D culture of primary human dermal fibroblasts in Matrigel. I tried working on different concentration variants of Matrigel- Normal (Basement membrane...

08 January 2019 8,248 3 View

How to maintain the level of Matrigel loaded cells in 3D culture using Microfluidic Chip?

I am using Matrigel for 3D organotypic co-culture of mammalian cells on microfluidic chip. I am using thick gel protocol of Matrigel for the same in order to entrap the cells. Initially I had...

04 August 2017 3,907 0 View

Is it possible to coat Matrigel (containning cell suspension) by thick gel protocol on wetted porous membrane?

I am working with establishment of 3D culture of mammalian cells on porous membrane (0.4 micron pore size) using Matrigel. I am using thick gel protocol for the same. Considering the conditions I...

09 February 2016 4,353 2 View