Manikanta Murahari

5 Questions 6 Answers 0 Followers

Questions related from Manikanta Murahari

Effect of solvents- Ethanol vs DMSO in in vitro cytotoxicity evaluation by SRB assay?

We are planning to screen synthetic compounds for in vitro cytotoxicity by SRB assay method. All the compounds are soluble in ethanol and DMSO. Can we screen the compounds by dissolving in...

08 August 2016 8,693 3 View

Can anyone suggest a protocol for MTT or SRB assay of nanoformulation?

We have prepared a nanoformulation of anticancer drug and performed MTT and SRB assay of API, nanoformulation and placebo (nanoformulation without drug) at concentrations of 5-100 ug/ml. OD...

02 February 2015 167 9 View

Can anyone please share P-gp overexpressing A549 cell line or MCF-7/ADR, which is renamed as NCI/ADR-RES cell line?

I have synthesized P-gp substrates and intend to evaluate MDR reversal activity on cancer resistant cell lines in combination with doxorubicin. I have checked with NCCS, Pune. It is not available...

01 January 2015 8,399 1 View

Which type of molecules will have more binding affinity to AT pairs and which type to GC pairs of DNA?

Can any structural features be identified for the binding affinity and what could be the reason for that?

05 May 2014 2,099 2 View

What makes the solubility of any compound decrease on cooling and increases on heating? Can anybody explain?

For example in water, why is solubility of compound less in ice water? Can anybody explain the difference between the nature of bonding in hot and cold water?

02 February 2013 4,916 2 View