8 Questions 17 Answers 0 Followers
Questions related from Mangesh Bawankar
Molecular weight calculate from primary structure of my protein is 23121 but after MALDI-TOF i got 22908.90 a difference of approximately 213 dalton. Any suggestions for the observed mass difference.
23 October 2018 3,765 3 View
I have my protein in the 1M NaoH solution. Protein has N terminal His Tag 1. I want to confirm, under this condition my protein will be in denature state. 2. If yes, then how do I refold my...
02 January 2017 1,461 12 View
I need to use AUC for my protein sample..My protein is in 20mM phosphate buffer + 100mM Nacl + 0.01% sodium azide. Can anyone tell me what is the sample requirement for analysis.
08 September 2016 2,094 2 View
I have performed gel filtration (superdex 75) and anion exchange chromatography for my protein. Solvent used for gel filtration is 20mM PB + 100mM nacl (ph 6.5) and for cation exchange, solvent A...
29 August 2016 3,366 12 View
i run 10% native gel, as per protocol given in molecular cloning (sambrook russell book) for SDS-PAGE, except SDS and Beta ME in loading dye and staking and running gel. But could not succeed. A...
19 August 2016 2,235 8 View
I have a mixture of monomer and dimer. Monomer of the protein has N terminal His tag and i did not remove the his tag after purification. I provide particular condition to my monomers so that two...
21 July 2016 5,414 3 View
i want to record the emission spectra of unknown compound. To do this i have record the absorbance of the compound, used the lamda max as the excitation wavelength and recorded the emission...
23 February 2015 9,445 7 View
Can anyone tell how do I concentrate the protein in the dialysis bag by using PEG?The molecular weight of my protein is 20 kD the dialysis cut of is 14 kD.
24 January 2015 4,468 11 View
