13 Questions 22 Answers 0 Followers
Questions related from Mangesh Bawankar
Molecular weight calculate from primary structure of my protein is 23121 but after MALDI-TOF i got 22908.90 a difference of approximately 213 dalton. Any suggestions for the observed mass difference.
23 October 2018 3,881 3 View
My protein form dimer trimer and tetramer, these species forms as a result of disulfide bond formation from monomer. Confirmed by nonreducing and reducing SDS PAGE. My protein monomer contains 6...
07 February 2017 8,337 4 View
I am using 40uM of the tht for the detection of amyloid. How to know this is the optimum concentration of tht for my assay.
19 January 2017 3,776 3 View
I have my protein in the 1M NaoH solution. Protein has N terminal His Tag 1. I want to confirm, under this condition my protein will be in denature state. 2. If yes, then how do I refold my...
02 January 2017 1,554 12 View
I need to use AUC for my protein sample..My protein is in 20mM phosphate buffer + 100mM Nacl + 0.01% sodium azide. Can anyone tell me what is the sample requirement for analysis.
08 September 2016 2,156 2 View
I have performed gel filtration (superdex 75) and anion exchange chromatography for my protein. Solvent used for gel filtration is 20mM PB + 100mM nacl (ph 6.5) and for cation exchange, solvent A...
29 August 2016 3,490 12 View
i run 10% native gel, as per protocol given in molecular cloning (sambrook russell book) for SDS-PAGE, except SDS and Beta ME in loading dye and staking and running gel. But could not succeed. A...
19 August 2016 2,320 8 View
I have a mixture of monomer and dimer. Monomer of the protein has N terminal His tag and i did not remove the his tag after purification. I provide particular condition to my monomers so that two...
21 July 2016 5,512 3 View
i want to record the emission spectra of unknown compound. To do this i have record the absorbance of the compound, used the lamda max as the excitation wavelength and recorded the emission...
23 February 2015 9,500 7 View
Can anyone tell how do I concentrate the protein in the dialysis bag by using PEG?The molecular weight of my protein is 20 kD the dialysis cut of is 14 kD.
24 January 2015 4,556 11 View
I have tried with 20% PEG, 100mM acetate buffer and ammonium sulphate (100mM to 400mM), incubate at 17 degree overnight but crystal did not form.
01 November 2014 6,415 7 View
I want to calculate secondary structure of the proteins by FTIR. To do so I recorded the Primary spectra of my protein. In order to get more resolution of the peaks I am doing deconvolution...
19 October 2014 1,302 8 View
I am using ATR FTIR to study complex formation between protein and ligand (drug). i am using ATR at 2 cm-1 and 4 cm-1 resolution. I record the FTIR spectra of my protein, ligand and protein+...
13 September 2014 629 1 View
