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Questions related from Mandy Kain
A few of my male Rag 2 -/- BALB/c mice have gotten infections - bilobed masses on their hindquarters. Many of the mice do get rectal prolapses, but these infections have been occurring in mice...
01 January 2013 4,491 3 View
All the protocols I've found are for DNA plasmids but I don't know if that means it definitely wouldn't work for RNAi.
10 October 2012 9,740 2 View
I would like to culture splenocytes in order to expand their Treg population using TGF-B, CD3 antibodies, and CD28. All the protocols I've found are for pre-sorted T cells using an isolation kit...
09 September 2012 4,096 8 View
One of our mouse strains has developed large tumors in their necks that may come from either the mammary glands, the salivary glands, or the thyroid. Do you know of any markers specific for these...
06 June 2012 9,694 13 View
I'm trying to create a knockdown line using either viral or non-viral methods (depending on availability), but I've never done this before and have only used established cell lines. Does anyone...
05 May 2012 10,049 4 View
I'm using the Click-it Cell Reaction Buffer Kit with AHA azide and AlexaFluor 647 alkyne to measure protein synthesis in HeLa cells and I'm having trouble reducing the background. The protocol...
01 January 2012 9,124 0 View
