Manasa Kongot

4 Questions 4 Answers 0 Followers

Questions related from Manasa Kongot

What are the techniques and methods by which I can confirm that the protein (BSA) solution I have prepared has not denatured?

I need to study the interaction of my compound with BSA protein using simple UV, Fluorescence and IR techniques. Once I prepare the protein solution in buffer, how should I confirm that the...

09 September 2017 4,954 7 View

I want to measure the change induced by my samples(dissolved in DMSO)on the secondary structure of a protein BSA by using CD Spectroscopic technique.

The problem is that DMSO has a cut-off absorption below 270nm and the protein shows CD signal in the far-UV region of about 208nm and hence I cannot measure the signal properly because of the high...

09 September 2016 6,303 4 View

What is the maximum concentration (%) of THF and DMSO upto which protein will remain unaltered?

I have my samples out of which few dissolve in THF and few in DMSO. I want to do interaction study of these compounds with model protein like Bovine Serum Albumin. Upto what percentage of these...

08 August 2016 9,410 3 View

Can anybody suggest a simple and effective method to remove moisture from my sample other than the hot-air oven technique?

Organic sample in the solid form (Excluding hot-air oven technique and desiccator technique)

01 January 2016 9,196 7 View