Mamoun Ahram

2 Questions 11 Answers 0 Followers

Questions related from Mamoun Ahram

Transfection of MDA-MB-453 cells with siRNA or a miRNA mimic increases expression of gene targets!!! How come?

We have been working on breast MDA-MB-453 cells. We found that hormonal treatment increases expression of three genes: Slug, RGS2 (regulator of G protein signaling 2), and MMP-13. When cell were...

04 April 2018 9,786 6 View

Why do aggregates appear prior to loading protein samples on gel for electrophoresis?

Fourty ug of lysed proteins are mixed with 5x loading dye (sample buffer) and boiled for 5 min. After cooling down the sample at RT and prior to loading, the sample appears as aggregates/cluster...

06 June 2013 7,136 7 View