Malgorzata Majewska

7 Questions 11 Answers 0 Followers

Questions related from Malgorzata Majewska

How can I analyze qPCR data without control sample/calibrator?

I would like to compare expression of my gene of interest (GOI) in non-treated leaves, stems, roots of my plant. In that case I don't have any control sample here and it's not possible to have...

10 August 2020 4,287 3 View

How should I calculate expression?

I would like to compare expression of my gene of interest (GOI) between different parts of my dicot plant (leaves, stems, roots). The plant parts are non-treated only (they are just like...

08 August 2020 4,547 3 View

Is it possible that dicot plant could rearrange or excise from his genome transfected DNA fragment?

I have transfected Salvia milthiorrhiza plant using Agrobacterium tumefaciens cells carrying binary plasmid. At the level of Agrobacterium I confirmed presence of my binary vector by obtaining...

27 June 2020 8,964 15 View

RNeasy PowerPlant Kit, Qiagen vs NucleoSpin RNA Plant and Fungi, Macherey-Nagel?

Which of the two kits is better for RNA isolation from plant rich in secondary metabolites? Maybe you could recommend me something else? I would like to obtain high quality and quantity RNA for...

18 November 2019 2,554 3 View

Which kit would you recommend for isolation of RNA from dicotyledonous plants (leaves, stems and roots)? Which vendor would you recommend generally?

My plant material is rich in secondary metabolites. With the isolated RNA I would like to examine expression of interested genes in Real Time PCR.

13 November 2019 5,518 8 View

Why I did not obtain expression after plant transformation?

I am studying some plant promoter activity. For this purpose I used commercial plasmid to which I inserted examined promoter sequence. The sequence was inserted above two fused marker genes: Gus...

10 June 2019 3,511 1 View

Which Kozak sequence should I use to obtain high protein expression in plants, especially in dicot Salvia milthiorrhiza?

question as above

19 October 2018 284 2 View