Malachi A Blundon

4 Questions 5 Answers 0 Followers

Questions related from Malachi A Blundon

Is there an enzymatic assay to test the metabolic activity of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB)?

I'd like to test the enzymatic function of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB) in cell culture after knocking down the PFKFB gene using shRNA. However I have yet to find...

04 April 2019 6,521 6 View

Which is more abundant: The proteome or the metabolome?

Considering the total contents of a cell or tissue lysate (of your liking), would the lysate contain more proteins or metabolites? I have an impression it contains more protein than metabolites....

10 October 2016 5,337 4 View

Not-so-typical troubleshooting considerations for S2 cell protein expression?

HI everyone, We've recently starting doing S2 cell work in our lab again and we have been having some issues getting transient transfections to work. We are working with standard S2 cells (not...

06 June 2016 3,188 1 View

Has anyone seen increase transcript production from fly mutants generated from p-element mobilization?

I have a mutant fly line that was generated by inserting a p-element in the 5' UTR of my gene. My RT-PCR suggests the transcript is elevated in my mutant. I am confident in my controls; they look...

06 June 2015 865 5 View