Maksym Plachynta

4 Questions 5 Answers 0 Followers

Questions related from Maksym Plachynta

Can Ab-magnetic beads used for MACS hinder further detection of antigen with flow cytometry?

Hellow everyone! I am using eBiosciense magnetic beads kit for MACS separation of murine CD11b+ cells (= monocytes and granulocytes). It consists of Biotin-labelled anti-CD11b Ab; and a secondary...

05 November 2015 1,360 6 View

Why do I get these autofluorescence peaks in in flow cytometry?

Hi all, I've just found something interesting about autofluorescence in flow cytometry. I've found, that autofluorescence, especially on FL1 [ex 488 / em 533] and FL2 [ex 488 / em 585] channels is...

16 September 2015 8,333 3 View

Is it a good idea to inject DMSO-dissolved drugs to mice intraperitoneally?

Hello everybody! I've just started a new series of experiments where I administer seferal DMSO-dissolved drugs to mice intraperitoneally (i.e. by injection into abdominal cavity), at 100 uL...

07 September 2015 7,823 10 View

How can EDTA affect flow cytometry results?

how can EDTA (added to trypsine when deattaching cells to remove calcium) affect flow cytometry results? does it bind to surface proteins and hinders Ab-Ag bunding?

11 July 2015 8,102 4 View