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Questions related from Maksym Plachynta
In mouse spleen, several populations of cells can be found by FCM. As far as I know, CD11b+Gr1+ ones are mature neutrophils and immature monocytes/macrophages; CD11b+Gr1- are mature...
19 November 2015 3,195 3 View
Hellow everyone! I am using eBiosciense magnetic beads kit for MACS separation of murine CD11b+ cells (= monocytes and granulocytes). It consists of Biotin-labelled anti-CD11b Ab; and a secondary...
05 November 2015 1,420 6 View
Hi all, I've just found something interesting about autofluorescence in flow cytometry. I've found, that autofluorescence, especially on FL1 [ex 488 / em 533] and FL2 [ex 488 / em 585] channels is...
16 September 2015 8,411 3 View
Hello everybody! I've just started a new series of experiments where I administer seferal DMSO-dissolved drugs to mice intraperitoneally (i.e. by injection into abdominal cavity), at 100 uL...
07 September 2015 7,866 10 View
how can EDTA (added to trypsine when deattaching cells to remove calcium) affect flow cytometry results? does it bind to surface proteins and hinders Ab-Ag bunding?
11 July 2015 8,162 4 View
