Mairaj Siddiquei

2 Questions 6 Answers 0 Followers

Questions related from Mairaj Siddiquei

A regular fluffy thick band at 60-70 kDa is absent in western blot samples for human vitreous, why so ??

Hi, I am frequently working with human vitreous samples to perform western blotting. Most of the time, I prepare a fresh sample, heat and load them on the same day. By this way, I gets a regular...

10 October 2017 5,979 2 View

In western blotting, recently, I am using std marker (Santa Cruz; sc-2361). For 10% SDS-PAGE gel, first top 4 bands are with more gaps - any suggestions?

Generally other standard markers, like from Biorad or invitrogen, the first 2-3 bands are closer in 10% and again last 2 bands are close and this band distribution gives the location of b-actin...

05 May 2013 9,038 3 View