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Questions related from Mahmoud Kamal Ahmadi
I am working with seven strains of Keio collection, but I have hard time to remove Kanamycin marker from one of those, particularly the one with Delta TyrA. I simply removed kan from the rest six...
01 January 2016 7,201 8 View
We need to construct orpon of 6 tandem genes (total 5 kbp) for protein expression in E.coli. We have previously constructed this opron using gnomic DNA and subcloning but we were not pleased with...
08 August 2015 5,895 4 View
I should display bunch of proteins on surface of E. coli. I have read “Ice Nucleation Protein” have been used as fusion tag frequently for this purpose. Any suggestions and opinions on possible...
10 October 2013 3,915 6 View