Mahmoud Kamal Ahmadi

3 Questions 10 Answers 0 Followers

Questions related from Mahmoud Kamal Ahmadi

Problem to remove Kanamycin marker from a Keio strain, any help?

I am working with seven strains of Keio collection, but I have hard time to remove Kanamycin marker from one of those, particularly the one with Delta TyrA. I simply removed kan from the rest six...

01 January 2016 7,205 8 View

Single gene synthesis or whole opron synthesis for protein expression in E.coli?

We need to construct orpon of 6 tandem genes (total 5 kbp) for protein expression in E.coli. We have previously constructed this opron using gnomic DNA and subcloning but we were not pleased with...

08 August 2015 5,898 4 View

E. coli protein surface display - can anyone help?

I should display bunch of proteins on surface of E. coli. I have read “Ice Nucleation Protein” have been used as fusion tag frequently for this purpose. Any suggestions and opinions on possible...

10 October 2013 3,917 6 View