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Questions related from Mahmoud Hefny Gad
Currently I am working with alpha amylase assay using DNS method but I have a problem with the colour which is not changing even I used high concentration from the phenolic standards (Trolox and...
19 August 2015 7,237 12 View
There several assays to measure the antioxidant activity, I performed Oxgyen Radical Absorbance Capacity (ORAC) with four concentration of Trolox (6.25-50 µM) and four sample concentrations. I...
09 June 2015 1,096 5 View
I tried to quantify the total flavonoid content in I using the NaNO2, ALCl3 and NaOH method with Catechin as a standard. I got a pink/red color with standard but i don't get the same color with my...
10 January 2015 4,312 7 View
I am working with a herbal plants and i wish to do ORAC assay for my samples to evaluate the antioxidant activity. Indeed, my problem is i don't know what is the suitable concentration should i...
08 January 2015 9,411 2 View
I want to know how can i remove the chlorophyll from my sample which was eluted with dichloromethane:ethyl acetate (3:1) from silica gel flash column. I used n-hexane as a first solvent in the...
28 March 2014 10,023 7 View
