7 Questions 16 Answers 0 Followers
Questions related from Mahdi Ghanbari
We are going to prepare a workstation for NGS data analysis. We wanna work with GS FLX+ and Illumina HiSeq 2500 data obtained from gut microbiota of animals. While some part is just 16s rDNA data,...
06 June 2014 4,428 5 View
In some articles I have seen that for screening of a specific gene the authors just used a primer set and then sequenced the fragment. But, in other articles, after PCR, fragments were cloned and...
05 May 2014 260 4 View
I am trying to amplify part of the 16S bacterial gene. I would really appreciate some advice. Running the PCR product on the gel, I obtained one specific band at the desired size and another...
03 March 2014 1,352 20 View
I am trying to amplify part of the 16S bacterial gene. I would really appreciate some advice. I used 59 degrees centigrade for annealing temp. Running the PCR product on the gel, I obtained one...
12 December 2013 7,243 23 View
Applying the so-called GS FLX++ technology for 454, some company claims that can now deliver approx one million reads with max single read length of up to 1100 base pairs (bp), resulting in up to...
11 November 2013 7,459 1 View
Any ideas on the effective and economical method for total DNA extraction from intestinal contents in fish?
10 October 2013 8,938 7 View
What is the appropriate way to preserve fish intestine samples for one or two months for bacteriological studies ? Do I need to add Glycerol to the samples and keep them at -20 degrees C?
09 September 2013 6,435 10 View
