Maggie Au

4 Questions 7 Answers 0 Followers

Questions related from Maggie Au

Different primer/probe concentration in multiplex qPCR

I have seen some papers, indicating they they have used different primer/probe concentrations in their multiplex qPCR experiment. For example, 900nM forward primer, 300nM reverse primer, 100mM...

19 May 2017 2,926 2 View

What is relative standard curve method in qPCR?

Can anyone explain what is relative standard curve method? How it is conducted? And how is that different from absolute standard curve method?

17 February 2017 457 2 View

What are these speckles in a PDMS microfluidic chip?

I've made a microfluidic chip using standard photolithography (SU8 photoresist). Cleaned with O2 plasma treatment and methanol for alignment. I used it by flowing some filtered DI water with beads...

12 April 2016 598 9 View

Why would two peptides have the same molecular weight but different HPLC retention times?

I have made a peptide using Fmoc SPPS and ran a small sample of that down an RP-HPLC. I saw two separable peaks (about 1 min apart) and then I tried to ID the two peaks using MS (ESI-MS). Both...

15 January 2014 2,830 19 View