Magdalena Wujak

3 Questions 15 Answers 0 Followers

Questions related from Magdalena Wujak

Can size exclusion chromatography and photo-induced crosslinking of unmodified proteins give different results from protein oligomerization studies?

Hi, Everyone, I performed oligomerization studies on my protein by applying two methods: size exclusion chromatography (SEC) and photo-induced crosslinking of unmodified proteins (PICUP). The...

06 June 2017 1,890 8 View

What is an efficient procedure for isolation of plasma membrane proteins from cultured animal cells ?

Hi everybody, I would like to check wheather my protein is located in plasma membrane. Can anyone recommend an efficient procedure for isolation of plasma membrane proteins from animal cells...

11 November 2015 4,452 4 View

Is it possible to express BRP gene (Bacteriocin Release Protein) carried by pSW1 plasmid in E. coli BL21 (DE3) host strain?

Hi everyone, has anybody worked with BRP-plasmids (from MoBiTec)? They carry a gene encoding BR protein (Bacteriocin Release Protein) enabling the secretion of a recombinant protein of interest to...

10 October 2015 6,435 2 View